Coding

Part:BBa_K1175005:Design

Designed by: Nicholas K. Goldner   Group: iGEM13_WLC-Milwaukee   (2013-09-27)


endo-1,4-beta-xylanase xynA from Bacillus Subtilis Subtilis 168


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 85
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 489
    Illegal SapI.rc site found at 543


Design Notes

A second stop codon was added.


Source

his gene has been isolated from the bacterium Bacillus subtilis subtilis 168

References